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Transcriptome Analysis

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Whole transcriptome seq. & analysis

Overview

Whole transcriptome seq. & analysis is a high-throughput sequencing technology for cDNA sequence used to obtain information of RNA contents of a sample. This technique has been used for research of diseases including cancer. NGS which provides deep coverage and high resolution (base-level), enables the detection of genes or alleles that are differentially expressed under certain circumstances. In addition, This technique can also provide information for functional RNAs that are not translated into proteins (non-coding RNA), post-transcriptional mutation, error correction and gene fusion.

Workflow

Characteristics

STRENGTH
Higher sensitivity than micro array
High resolution (base-level)
Do not require prior knowledge of the organism
OPPORTUNITY
DEG research
Differentially expressed alleles research
Non-translated RNA research
Post-transcriptional mutation/error correction research
Gene fusion research

mRNA seq. & analysis

Overview

mRNA seq. & analysis is a high-throughput sequencing technology for cDNA sequence used to obtain information of RNA contents of a sample. This technique has been used for research of diseases including cancer. NGS which provides deep coverage and high resolution (base-level), enables the detection of genes or alleles that are differentially expressed under certain circumstances. In addition, This technique can also provide information for functional RNAs that are not translated into proteins (non-coding RNA), post-transcriptional mutation, error correction and gene fusion.

Workflow

Characteristics

STRENGTH
Higher sensitivity than micro array
High resolution (base-level)
Do not require prior knowledge of the organism
Various types of analysis available
OPPORTUNITY
DEG research
Differentially expressed alleles research
Non-translated RNA research
Post-transcriptional mutation/error correction research
Gene fusion research

smallRNA seq. & analysis

Overview

SmallRNA seq. & analysis is distinct from other RNA sequencing methods as its main targets are smallRNAs. Research on tissue specific expression of previously identified smallRNAs, association with diseases, and isoform are now possible as well as the detection of unknown small RNAs. It also suggests potential of miRNAs which are known to cause abnormal functioning for an organism in activity and chemical level, to serve as an important tool for prediction or diagnosis of diseases including cancer in the future.

Workflow

Characteristics

STRENGTH
Higher sensitivity than micro array
High resolution (base-level)
Whole genomic approach
OPPORTUNITY
Research on tissue specific expression pattern of smallRNA
Research on smallRNA association with disease
SmallRNA subtype research
Screening of new smallRNA
Research on smallRNAs that cause abnormal function in diseases

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